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rat anti myelin basic protein  (Bio-Rad)


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    Structured Review

    Bio-Rad rat anti myelin basic protein
    Rat Anti Myelin Basic Protein, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 248 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rat+anti+myelin+basic+protein+mbp/Rat+anti+MBP+(aa82-87)/pmc13089494-166-31-36
    Average 96 stars, based on 248 article reviews
    rat anti myelin basic protein - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Immunohistochemistry:

    Article Title: Transcriptional profiles of mouse oligodendrocyte precursor cells across the lifespan.
    Article Snippet: Oligodendrocyte progenitor cells (OPCs) are highly dynamic, widely distributed glial cells of the central nervous system responsible for generating myelinating oligodendrocytes throughout life.. However, the rates of OPC proliferation and differentiation decline dramatically with aging, which may impair homeostasis, remyelination and adaptive myelination during learning.. To determine how aging influences OPCs, we generated a transgenic mouse line (Matn4-mEGFP) and performed single-cell RNA sequencing, providing enhanced resolution of transcriptional changes during key transitions from quiescence to proliferation and differentiation across the lifespan.

    In Vitro:

    Article Title: Transcriptional profiles of mouse oligodendrocyte precursor cells across the lifespan.
    Article Snippet: Oligodendrocyte progenitor cells (OPCs) are highly dynamic, widely distributed glial cells of the central nervous system responsible for generating myelinating oligodendrocytes throughout life.. However, the rates of OPC proliferation and differentiation decline dramatically with aging, which may impair homeostasis, remyelination and adaptive myelination during learning.. To determine how aging influences OPCs, we generated a transgenic mouse line (Matn4-mEGFP) and performed single-cell RNA sequencing, providing enhanced resolution of transcriptional changes during key transitions from quiescence to proliferation and differentiation across the lifespan.

    Immunofluorescence:

    Article Title: Transcriptional profiles of mouse oligodendrocyte precursor cells across the lifespan.
    Article Snippet: Oligodendrocyte progenitor cells (OPCs) are highly dynamic, widely distributed glial cells of the central nervous system responsible for generating myelinating oligodendrocytes throughout life.. However, the rates of OPC proliferation and differentiation decline dramatically with aging, which may impair homeostasis, remyelination and adaptive myelination during learning.. To determine how aging influences OPCs, we generated a transgenic mouse line (Matn4-mEGFP) and performed single-cell RNA sequencing, providing enhanced resolution of transcriptional changes during key transitions from quiescence to proliferation and differentiation across the lifespan.

    SDS Page:

    Article Title: In vivo molecular signatures of cerebellar pathology in spinocerebellar ataxia type 3
    Article Snippet: Supernatants (soluble protein fractions) were collected and total protein concentration was determined using the BCA method (Pierce). .. Total protein lysates from soluble fractions were resolved on 10% SDS-PAGE gels, and corresponding PVDF membranes were incubated overnight at 4 oC with primary antibodies: rat anti-myelin basic protein (MBP) (1:1000; MCA409S, AbD Serotec), mouse anti-160 kD neurofilament medium (NFL) (1:1000; ab7794, Abcam), rabbit anti-α-tubulin (1:5000; 2144S, Cell Signaling), mouse anti-GAPDH (1:10000; MAB374, Millipore), and rabbit anti-MJD (1:20000). .. Bound primary antibodies were visualized by incubation with a peroxidase-conjugated anti-rat, anti-mouse or anti-rabbit secondary antibody (1:10000; Jackson Immuno Research Laboratories) followed by treatment with ECL-plus reagent (Western Lighting, PerkinElmer) and exposure to autoradiography films.

    Incubation:

    Article Title: In vivo molecular signatures of cerebellar pathology in spinocerebellar ataxia type 3
    Article Snippet: Supernatants (soluble protein fractions) were collected and total protein concentration was determined using the BCA method (Pierce). .. Total protein lysates from soluble fractions were resolved on 10% SDS-PAGE gels, and corresponding PVDF membranes were incubated overnight at 4 oC with primary antibodies: rat anti-myelin basic protein (MBP) (1:1000; MCA409S, AbD Serotec), mouse anti-160 kD neurofilament medium (NFL) (1:1000; ab7794, Abcam), rabbit anti-α-tubulin (1:5000; 2144S, Cell Signaling), mouse anti-GAPDH (1:10000; MAB374, Millipore), and rabbit anti-MJD (1:20000). .. Bound primary antibodies were visualized by incubation with a peroxidase-conjugated anti-rat, anti-mouse or anti-rabbit secondary antibody (1:10000; Jackson Immuno Research Laboratories) followed by treatment with ECL-plus reagent (Western Lighting, PerkinElmer) and exposure to autoradiography films.

    Binding Assay:

    Article Title: A macro-transection model of brain trauma for neuromaterial testing with functional electrophysiological readouts
    Article Snippet: After 3× PBS washes, coverslips were mounted on glass slides with Vectashield® mounting medium containing 4′,6-diamidino-2-phenylindole (DAPI) nuclear stain (Cat# H-1200, Vector Laboratories, Burlingame, CA, USA). .. Primary antibodies were rabbit anti-glial fibrillary acidic protein (GFAP) (1:500, Cat# Z0334, Santa Cruz Biotechnology, Heidelberg, Germany), mouse anti-β-tubulin (Tuj-1) (1:500, Cat# 801202, BioLegend, San Diego, CA, USA), goat anti-ionized calcium binding adaptor molecule 1 (Iba1) (1:500, Cat# ab5076, Abcam, Cambridge, UK), mouse anti-neuronal glial antigen2 (NG2) (1:500, Cat# ab275024, Abcam), rat anti-myelin basic protein (MBP) (1:200) (Cat# 160223, BioRad, Hercules, CA, USA). .. Secondary antibodies were fluorescein isothiocyanate (FITC)-conjugated donkey anti-mouse, -rabbit, -goat; cyanine 3 (Cy3)-conjugated donkey anti-mouse, -goat, -rat; cyanine 5 (Cy5)-conjugated and were incubated at 1:200 (Cat# 711-165-151, Cat# 711-165-152, Cat# 711-165-153, Cat# 711-095-151, Cat# 711-095-152, and Cat# 711-095-153, Stratech Scientific, Suffolk, UK).



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